This thesis work is dedicated to the design, development and characterization of Micro-Pattern Gas Detectors. The performances of a Time Projection Chamber (TPC) equipped with a triple Gas Electron Multiplier (GEM) amplification structure are reported. The intrinsic ion backflow suppression of GEM foils drastically reduces the space charge produced by wire readout in traditional TPC. The GEM solution allows the operation of a TPC at much higher event rate. The second part of this thesis describes the development of a 40 × 40 cm2 Micromegas detector with a highly segmented central area. A reduction of discharges compared to conventional Micromegas detectors is needed for stable operation in intense beams of hadrons. Spark reduction technologies have been successfully studied and results are presented.